› 6g75

X-ray diffraction
1.39Å resolution

Crystal structure of the common ancestor of haloalkane dehalogenases and Renilla luciferase (AncHLD-RLuc)

Released:
DOI: 10.2210/pdb6g75/pdb
PDB entry 6g75 coloured by chain, front view.
PDB entry 6g75 coloured by chain, side view.
PDB entry 6g75 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Light-Emitting Dehalogenases: Reconstruction of Multifunctional Biocatalysts
Chaloupkova R, Liskova V, Toul M, Markova K, Sebestova E, Hernychova L, Marek M, Pinto GP, Pluskal D, Waterman J, Prokop Z, Damborsky J
ACS Catalysis (2019)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166767 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Common ancestor of haloalkane dehalogenase and Renilla luciferase (AncHLD-RLuc) Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 34.04 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand OXY 2 x OXY
Ligand MPD 2 x MPD
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 44.549Å b: 60.51Å c: 123.63Å
α: 90° β: 90.9° γ: 90°
R-values:
R R work R free
0.152 0.15 0.19
Expression system: Escherichia coli BL21(DE3)

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