› 6g80

X-ray diffraction
2.05Å resolution

Structure of Mycobacterium hassiacum MeT1 from orthorhombic crystals.

Released:
DOI: 10.2210/pdb6g80/pdb
PDB entry 6g80 coloured by chain, front view.
PDB entry 6g80 coloured by chain, side view.
PDB entry 6g80 coloured by chain, top view.
Source organism: Mycolicibacterium hassiacum DSM 44199
Primary publication:
Biosynthesis of mycobacterial methylmannose polysaccharides requires a unique 1-O-methyltransferase specific for 3-O-methylated mannosides.
Ripoll-Rozada J, Costa M, Manso JA, Maranha A, Miranda V, Sequeira A, Ventura MR, Macedo-Ribeiro S, Pereira PJB, Empadinhas N
Proc Natl Acad Sci U S A 116 835-844 (2019)
PMID: 30606802

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 3,3'-di-O-methyl-4-alpha-mannobiose = S-adenosyl-L-homocysteine + 1,3,3'-tri-O-methyl-4-alpha-mannobiose
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MMP 1-O-methyltransferase Chains: A, B, C, M
Molecule details ›
Chains: A, B, C, M
Length: 231 amino acids
Theoretical weight: 25.45 KDa
Source organism: Mycolicibacterium hassiacum DSM 44199
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K5B7F3 (Residues: 1-218; Coverage: 100%)
Gene names: C731_4163, MHAS_04405, meT1
Sequence domains: Methyltransferase domain

Ligands and Environments

2 bound ligands:
Ligand SAH 3 x SAH
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 117.15Å b: 119.72Å c: 145Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

S-Adenosylmethionine (SAM)-Dependent Methyltransferase MftM is Responsible for Methylation of the Redox Cofactor Mycofactocin.
Ellerhorst et al. (2022)
4 more

1 mention without citation

Biosynthesis of mycobacterial methylmannose polysaccharides requires a unique 1-O-methyltransferase specific for 3-O-methylated mannosides.
Ripoll-Rozada et al. (2019)