› 6g8e

X-ray diffraction
1.7Å resolution

Crystal Structure of the Amyloid-like VTQVGF segment from the R5 repeat of the E. coli Biofilm-associated CsgA Curli protein

Released:
DOI: 10.2210/pdb6g8e/pdb
PDB entry 6g8e coloured by chain, front view.
PDB entry 6g8e coloured by chain, side view.
PDB entry 6g8e coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural Insights into Curli CsgA Cross-β Fibril Architecture Inspire Repurposing of Anti-amyloid Compounds as Anti-biofilm Agents.
Perov S, Lidor O, Salinas N, Golan N, Tayeb-Fligelman E, Deshmukh M, Willbold D, Landau M
PLoS Pathog 15 e1007978 (2019)
PMID: 31469892

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major curlin subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 6 amino acids
Theoretical weight: 650 Da
Source organism: Escherichia coli K-12
Expression system: Not provided
UniProt:
  • Canonical: P28307 (Residues: 137-142; Coverage: 5%)
Gene names: JW1025, b1042, csgA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P1
Unit cell:
a: 4.81Å b: 19.34Å c: 21.9Å
α: 65.82° β: 83.72° γ: 83.2°
R-values:
R R work R free
0.124 0.123 0.132
Expression system: Not provided

Quick links

Citations

14 review citations

Biophysical processes underlying cross-seeding in amyloid aggregation and implications in amyloid pathology.
Ivanova et al. (2021)
13 more

3 mentions without citation

Interaction with the host: the role of fibronectin and extracellular matrix proteins in the adhesion of Gram-negative bacteria.
Vaca et al. (2020)
2 more