› 6g8r

X-ray diffraction
2.74Å resolution

SP140 PHD-Bromodomain complex with scFv

Released:
DOI: 10.2210/pdb6g8r/pdb
PDB entry 6g8r coloured by chain, front view.
PDB entry 6g8r coloured by chain, side view.
PDB entry 6g8r coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Fairhead M, Graslund S, Strain-Damerell C, Picaud SS, Pike ACW, Pinkas DM, Wigren E, Preger C, Persson Lotsholm H, Ossipova E, Filippakopoulos P, Burgess-Brown NA, Arrowsmith CH, Edwards AM, Bountra C, von Delft F, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212001 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
single-chain variable fragment Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 26.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Nuclear body protein SP140 Chain: B
Molecule details ›
Chain: B
Length: 198 amino acids
Theoretical weight: 23.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13342 (Residues: 687-862; Coverage: 20%)
Gene names: LYSP100, SP140
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P6122
Unit cell:
a: 89.2Å b: 89.2Å c: 343.35Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.215 0.249
Expression system: Escherichia coli

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