› 6g9q

X-ray diffraction
1.89Å resolution

Ternary complex of P14 TCR with murine MHC class I H-2 Db in complex with self-antigen derived from dopamine monooxygenase.

Released:
DOI: 10.2210/pdb6g9q/pdb
PDB entry 6g9q coloured by chain, front view.
PDB entry 6g9q coloured by chain, side view.
PDB entry 6g9q coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Achour A, Sandalova T, Allerbring E

Function and Biology Details

Reaction catalysed: 
3,4-dihydroxyphenethylamine + 2 ascorbate + O(2) = noradrenaline + 2 monodehydroascorbate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-101335 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
H-2 class I histocompatibility antigen, D-B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 32.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01899 (Residues: 25-300; Coverage: 82%)
Gene name: H2-D1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 119 amino acids
Theoretical weight: 13.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 1-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: G
Molecule details ›
Chain: G
Length: 205 amino acids
Theoretical weight: 23.03 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0G2JF94 (Residues: 22-120; Coverage: 99%)
Gene name: Trav14-1
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
T-cell receptor beta chain V region C5; T-cell receptor beta-1 chain C region Chain: H
Molecule details ›
Chain: H
Length: 238 amino acids
Theoretical weight: 26.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04213 (Residues: 11-122; Coverage: 97%)
  • Canonical: P01852 (Residues: 1-127; Coverage: 73%)
Sequence domains:
Structure domains: Immunoglobulins
Soluble dopamine beta-hydroxylase Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 1.04 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q64237 (Residues: 557-565; Coverage: 1%)
Gene name: Dbh

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 256.383Å b: 46.622Å c: 90.059Å
α: 90° β: 94.2° γ: 90°
R-values:
R R work R free
0.19 0.187 0.248
Expression systems:
  • Escherichia coli
  • Not provided

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