› 6gff

X-ray diffraction
3.1Å resolution

Structure of GARP (LRRC32) in complex with latent TGF-beta1 and MHG-8 Fab

Released:
DOI: 10.2210/pdb6gff/pdb
PDB entry 6gff coloured by chain, front view.
PDB entry 6gff coloured by chain, side view.
PDB entry 6gff coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of latent TGF-β1 presentation and activation by GARP on human regulatory T cells.
Liénart S, Merceron R, Vanderaa C, Lambert F, Colau D, Stockis J, van der Woning B, De Haard H, Saunders M, Coulie PG, Savvides SN, Lucas S
Science 362 952-956 (2018)
PMID: 30361387

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208350 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (8 distinct):
Latency-associated peptide Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 249 amino acids
Theoretical weight: 28.53 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01137 (Residues: 30-278; Coverage: 69%)
Gene names: TGFB, TGFB1
Sequence domains: TGF-beta propeptide
Transforming growth factor beta-1 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 112 amino acids
Theoretical weight: 12.81 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01137 (Residues: 279-390; Coverage: 31%)
Gene names: TGFB, TGFB1
Sequence domains: Transforming growth factor beta like domain
Transforming growth factor beta activator LRRC32 Chains: I, J
Molecule details ›
Chains: I, J
Length: 618 amino acids
Theoretical weight: 67.24 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q14392 (Residues: 20-628; Coverage: 95%)
Gene names: D11S833E, LRRC32
Sequence domains:
MHG-8 Fab light chain Chains: K, M
Molecule details ›
Chains: K, M
Length: 212 amino acids
Theoretical weight: 23.38 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
MHG-8 Fab heavy chain Chains: L, N
Molecule details ›
Chains: L, N
Length: 221 amino acids
Theoretical weight: 23.83 KDa
Source organism: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 103.86Å b: 175.01Å c: 145.77Å
α: 90° β: 92.23° γ: 90°
R-values:
R R work R free
0.235 0.233 0.268
Expression system: Homo sapiens

Quick links

Citations

28 review citations

TGFβ biology in cancer progression and immunotherapy.
Derynck et al. (2021)
27 more

10 mentions without citation

GARP: A Key Target to Evaluate Tumor Immunosuppressive Microenvironment.
Bouchard et al. (2021)
9 more