› 6gid

X-ray diffraction
1.9Å resolution

High resolution crystal structure of substrate-free human neprilysin

Released:
DOI: 10.2210/pdb6gid/pdb
PDB entry 6gid coloured by chain, front view.
PDB entry 6gid coloured by chain, side view.
PDB entry 6gid coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High resolution crystal structure of substrate-free human neprilysin.
Moss S, Subramanian V, Acharya KR
J Struct Biol 204 19-25 (2018)
PMID: 29906506

Function and Biology Details

Reaction catalysed: 
Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140129 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neprilysin Chain: A
Molecule details ›
Chain: A
Length: 696 amino acids
Theoretical weight: 79.53 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P08473 (Residues: 55-750; Coverage: 93%)
Gene names: EPN, MME
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand NAG 4 x NAG
Ligand ZN 1 x ZN
Ligand EDO 6 x EDO
Ligand NO3 2 x NO3
Ligand PEG 3 x PEG
Ligand GOL 1 x GOL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3221
Unit cell:
a: 107.424Å b: 107.424Å c: 112.316Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.235
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

HIV and Alzheimer's disease: complex interactions of HIV-Tat with amyloid β peptide and Tau protein.
Hategan et al. (2019)
1 more

7 mentions without citation

Molecular Basis for Omapatrilat and Sampatrilat Binding to Neprilysin-Implications for Dual Inhibitor Design with Angiotensin-Converting Enzyme.
Sharma et al. (2020)
6 more