› 6gje

X-ray diffraction
2.3Å resolution

Structure of the Amnionless(20-357)-Cubilin(36-135) complex

Released:
DOI: 10.2210/pdb6gje/pdb
PDB entry 6gje coloured by chain, front view.
PDB entry 6gje coloured by chain, side view.
PDB entry 6gje coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural assembly of the megadalton-sized receptor for intestinal vitamin B12 uptake and kidney protein reabsorption.
Larsen C, Etzerodt A, Madsen M, Skjødt K, Moestrup SK, Andersen CBF
Nat Commun 9 5204 (2018)
PMID: 30523278

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129996 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Soluble protein amnionless Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 35.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q9BXJ7 (Residues: 20-357; Coverage: 78%)
Gene names: AMN, UNQ513/PRO1028
Sequence domains: Amnionless
Cubilin Chains: B, C, D
Molecule details ›
Chains: B, C, D
Length: 110 amino acids
Theoretical weight: 12.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: O60494 (Residues: 26-135; Coverage: 3%)
Gene names: CUBN, IFCR

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I222
Unit cell:
a: 70.28Å b: 158.84Å c: 237.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.23
Expression system: Escherichia coli K-12

Quick links

Citations

3 review citations

Albumin uptake and processing by the proximal tubule: physiological, pathological, and therapeutic implications.
Molitoris et al. (2022)
2 more