› 6gk7

X-ray diffraction
2.95Å resolution

Crystal structure of anti-tau antibody dmCBTAU-27.1, double mutant (S31Y, T100I) of CBTAU-27.1, in complex with Tau peptide A8119B (residues 299-318)

Released:
DOI: 10.2210/pdb6gk7/pdb
PDB entry 6gk7 coloured by chain, front view.
PDB entry 6gk7 coloured by chain, side view.
PDB entry 6gk7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A common antigenic motif recognized by naturally occurring human VH5-51/VL4-1 anti-tau antibodies with distinct functionalities.
Apetri A, Crespo R, Juraszek J, Pascual G, Janson R, Zhu X, Zhang H, Keogh E, Holland T, Wadia J, Verveen H, Siregar B, Mrosek M, Taggenbrock R, Ameijde J, Inganäs H, van Winsen M, Koldijk MH, Zuijdgeest D, Borgers M, Dockx K, Stoop EJM, Yu W, Brinkman-van der Linden EC, Ummenthum K, van Kolen K, Mercken M, Steinbacher S, de Marco D, Hoozemans JJ, Wilson IA, Koudstaal W, Goudsmit J
Acta Neuropathol Commun 6 43 (2018)
PMID: 29855358

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210479 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HUMAN FAB ANTIBODY FRAGMENT OF CBTAU-27.1(S31Y,T100I) Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 24.12 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
HUMAN FAB ANTIBODY FRAGMENT OF CBTAU-27.1(S31Y,T100I) Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 23.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Microtubule-associated protein tau Chain: A
Molecule details ›
Chain: A
Length: 11 amino acids
Theoretical weight: 1.26 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P10636 (Residues: 625-635; Coverage: 2%)
Gene names: MAPT, MAPTL, MTBT1, TAU
Sequence domains: Tau and MAP protein, tubulin-binding repeat

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 93.829Å b: 60.524Å c: 91.048Å
α: 90° β: 104.37° γ: 90°
R-values:
R R work R free
0.224 0.22 0.268
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

1 review citation

Intersection of pathological tau and microglia at the synapse.
Vogels et al. (2019)
5 other articles

1 mention without citation

Insights into the Structural Conformations of the Tau Protein in Different Aggregation Status.
Pinzi et al. (2023)