› 6gmc

X-ray diffraction
1.2Å resolution

1.2 A resolution structure of human hydroxyacid oxidase 1 bound with FMN and 4-carboxy-5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole

Released:
DOI: 10.2210/pdb6gmc/pdb
PDB entry 6gmc coloured by chain, front view.
PDB entry 6gmc coloured by chain, side view.
PDB entry 6gmc coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: MacKinnon S, Bezerra GA, Krojer T, Smee C, Arrowsmith CH, Edwards E, Bountra C, Oppermann U, Brennan PE, Yue WW

Function and Biology Details

Reactions catalysed: 
(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2)
Glyoxylate + H(2)O + O(2) = oxalate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193816 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-Hydroxyacid oxidase 1 Chain: A
Molecule details ›
Chain: A
Length: 368 amino acids
Theoretical weight: 40.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UJM8 (Residues: 1-362; Coverage: 98%)
Gene names: GOX1, HAO1
Sequence domains: FMN-dependent dehydrogenase
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
2 bound ligands:
Ligand EDO 4 x EDO
Ligand C7C 1 x C7C
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I4
Unit cell:
a: 97.348Å b: 97.348Å c: 80.363Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.135 0.134 0.155
Expression system: Escherichia coli

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