› 6gmf

X-ray diffraction
1.55Å resolution

Structure of Cytochrome P450 CYP109Q5 from Chondromyces apiculatus

Released:
DOI: 10.2210/pdb6gmf/pdb
PDB entry 6gmf coloured by chain, front view.
PDB entry 6gmf coloured by chain, side view.
PDB entry 6gmf coloured by chain, top view.
Source organism: Chondromyces apiculatus DSM 436
Primary publication:
Characterization and structure-guided engineering of the novel versatile terpene monooxygenase CYP109Q5 from Chondromyces apiculatus DSM436.
Klenk JM, Dubiel P, Sharma M, Grogan G, Hauer B
Microb Biotechnol 12 377-391 (2019)
PMID: 30592153

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100022 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative cytochrome P450 hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 397 amino acids
Theoretical weight: 43.92 KDa
Source organism: Chondromyces apiculatus DSM 436
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A017T5A5 (Residues: 1-397; Coverage: 100%)
Gene name: CAP_4855
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 44.69Å b: 55.02Å c: 74.8Å
α: 90° β: 91.67° γ: 90°
R-values:
R R work R free
0.187 0.185 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Cytochrome P450 for environmental remediation: catalytic mechanism, engineering strategies and future prospects.
Yang et al. (2023)
2 other articles

1 mention without citation

Characterization and structure-guided engineering of the novel versatile terpene monooxygenase CYP109Q5 from Chondromyces apiculatus DSM436.
Klenk et al. (2019)