› 6go8

X-ray diffraction
1.65Å resolution

Structure of GFPmut2 crystallized at pH 6

Released:
DOI: 10.2210/pdb6go8/pdb
PDB entry 6go8 coloured by chain, front view.
PDB entry 6go8 coloured by chain, side view.
PDB entry 6go8 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Insight into GFPmut2 pH Dependence by Single Crystal Microspectrophotometry and X-ray Crystallography.
Lolli G, Raboni S, Pasqualetto E, Benoni R, Campanini B, Ronda L, Mozzarelli A, Bettati S, Battistutta R
J Phys Chem B 122 11326-11337 (2018)
PMID: 30179482

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 27.43 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand MPD 1 x MPD
Ligand MRD 3 x MRD
1 modified residue:
Ligand PIA 1 x PIA

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 32.504Å b: 60.009Å c: 112.497Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.174
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Mitochondrial Reactive Oxygen Species Generated at the Complex-II Matrix or Intermembrane Space Microdomain Have Distinct Effects on Redox Signaling and Stress Sensitivity in Caenorhabditis elegans.
Trewin et al. (2019)