› 6gpa

X-ray diffraction
1.79Å resolution

Function and Biology Details

Reaction catalysed: 
The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183565 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arabinogalactan endo-beta-1,4-galactanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 35.43 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q89YR3 (Residues: 38-351; Coverage: 95%)
Gene name: BT_4668
Sequence domains: Glycosyl hydrolase family 53

Ligands and Environments

1 bound ligand:
Ligand GAL 2 x GAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21
Unit cell:
a: 47.544Å b: 45.632Å c: 138.76Å
α: 90° β: 99.8° γ: 90°
R-values:
R R work R free
0.173 0.17 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 citation in other articles

Structural Identity of Galactooligosaccharide Molecules Selectively Utilized by Single Cultures of Probiotic Bacterial Strains.
Böger et al. (2019)
6 more

5 mentions without citation

High pressure and multiferroics materials: a happy marriage.
Gilioli et al. (2014)
4 more