› 6grw

X-ray diffraction
1.5Å resolution

Glucuronoyl Esterase from Opitutus terrae (Au derivative)

Released:
DOI: 10.2210/pdb6grw/pdb
PDB entry 6grw coloured by chain, front view.
PDB entry 6grw coloured by chain, side view.
PDB entry 6grw coloured by chain, top view.
Source organism: Opitutus terrae PB90-1
Primary publication:
Biochemical and structural features of diverse bacterial glucuronoyl esterases facilitating recalcitrant biomass conversion.
Arnling Bååth J, Mazurkewich S, Knudsen RM, Poulsen JN, Olsson L, Lo Leggio L, Larsbrink J
Biotechnol Biofuels 11 213 (2018)
PMID: 30083226

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative acetyl xylan esterase Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 43.99 KDa
Source organism: Opitutus terrae PB90-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1ZMF4 (Residues: 33-432; Coverage: 96%)
Gene name: Oter_0116

Ligands and Environments

5 bound ligands:
Ligand AU 2 x AU
Ligand CA 1 x CA
Ligand MG 2 x MG
Ligand MPD 1 x MPD
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P1
Unit cell:
a: 43.693Å b: 44.608Å c: 50.558Å
α: 76.573° β: 67.172° γ: 70.648°
R-values:
R R work R free
0.136 0.134 0.158
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

A Review on Bacterial Contribution to Lignocellulose Breakdown into Useful Bio-Products.
Chukwuma et al. (2021)
3 more

2 mentions without citation

Glucuronoyl esterases - enzymes to decouple lignin and carbohydrates and enable better utilization of renewable plant biomass.
Larsbrink et al. (2023)
1 more