› 6gs0

X-ray diffraction
1.34Å resolution

Native Glucuronoyl Esterase from Opitutus terrae

Released:
DOI: 10.2210/pdb6gs0/pdb
PDB entry 6gs0 coloured by chain, front view.
PDB entry 6gs0 coloured by chain, side view.
PDB entry 6gs0 coloured by chain, top view.
Source organism: Opitutus terrae PB90-1
Primary publication:
Biochemical and structural features of diverse bacterial glucuronoyl esterases facilitating recalcitrant biomass conversion.
Arnling Bååth J, Mazurkewich S, Knudsen RM, Poulsen JN, Olsson L, Lo Leggio L, Larsbrink J
Biotechnol Biofuels 11 213 (2018)
PMID: 30083226

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative acetyl xylan esterase Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 43.99 KDa
Source organism: Opitutus terrae PB90-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1ZMF4 (Residues: 33-432; Coverage: 96%)
Gene name: Oter_0116

Ligands and Environments

4 bound ligands:
Ligand EDO 9 x EDO
Ligand PGE 1 x PGE
Ligand SO4 1 x SO4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P1
Unit cell:
a: 43.361Å b: 44.263Å c: 50.027Å
α: 75.863° β: 66.326° γ: 70.876°
R-values:
R R work R free
0.174 0.173 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

A Review on Bacterial Contribution to Lignocellulose Breakdown into Useful Bio-Products.
Chukwuma et al. (2021)
4 more

6 mentions without citation

Biochemical and structural features of diverse bacterial glucuronoyl esterases facilitating recalcitrant biomass conversion.
Arnling Bååth et al. (2018)
5 more