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X-ray diffraction
1.7Å resolution

Siderophore hydrolase EstB mutant H267N from Aspergillus fumigatus

Released:
DOI: 10.2210/pdb6gui/pdb
PDB entry 6gui coloured by chain, front view.
PDB entry 6gui coloured by chain, side view.
PDB entry 6gui coloured by chain, top view.
Source organism: Aspergillus fumigatus Af293
Primary publication:
Iron Scavenging in Aspergillus Species: Structural and Biochemical Insights into Fungal Siderophore Esterases.
Ecker F, Haas H, Groll M, Huber EM
Angew Chem Int Ed Engl 57 14624-14629 (2018)
PMID: 30070018

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175813 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Siderophore triacetylfusarinine C esterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 292 amino acids
Theoretical weight: 32.65 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4WF29 (Residues: 3-292; Coverage: 99%)
Gene names: AFUA_3G03660, estB
Sequence domains: Putative esterase

Ligands and Environments

1 bound ligand:
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 54.97Å b: 92.61Å c: 130.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.187
Expression system: Escherichia coli

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Citations

1 review citation

Siderophore-Based Molecular Imaging of Fungal and Bacterial Infections-Current Status and Future Perspectives.
Petrik et al. (2020)
4 other articles