› 6gul

X-ray diffraction
2.3Å resolution

Siderophore hydrolase EstB mutant E211Q from Aspergillus fumigatus

Released:
DOI: 10.2210/pdb6gul/pdb
PDB entry 6gul coloured by chain, front view.
PDB entry 6gul coloured by chain, side view.
PDB entry 6gul coloured by chain, top view.
Source organism: Aspergillus fumigatus Af293
Primary publication:
Iron Scavenging in Aspergillus Species: Structural and Biochemical Insights into Fungal Siderophore Esterases.
Ecker F, Haas H, Groll M, Huber EM
Angew Chem Int Ed Engl 57 14624-14629 (2018)
PMID: 30070018

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175813 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Siderophore triacetylfusarinine C esterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 292 amino acids
Theoretical weight: 32.67 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4WF29 (Residues: 3-292; Coverage: 99%)
Gene names: AFUA_3G03660, estB
Sequence domains: Putative esterase

Ligands and Environments

3 bound ligands:
Ligand GOL 3 x GOL
Ligand SO4 3 x SO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P212121
Unit cell:
a: 54.67Å b: 91.83Å c: 130.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.251
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Siderophore-Based Molecular Imaging of Fungal and Bacterial Infections-Current Status and Future Perspectives.
Petrik et al. (2020)
4 other articles