› 6gv3

X-ray diffraction
1.2Å resolution

Structure of the E2 conjugating enzyme, SCE1, from Arabidopsis thaliana.

Released:
DOI: 10.2210/pdb6gv3/pdb
PDB entry 6gv3 coloured by chain, front view.
PDB entry 6gv3 coloured by chain, side view.
PDB entry 6gv3 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural insights into SUMO E1-E2 interactions in Arabidopsis uncovers a distinctive platform for securing SUMO conjugation specificity across evolution.
Liu B, Lois LM, Reverter D
Biochem J 476 2127-2139 (2019)
PMID: 31292170

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174967 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SUMO-conjugating enzyme SCE1 Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 20.18 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q42551 (Residues: 1-160; Coverage: 100%)
Gene names: AHUS5, At3g57870, EMB1637, SCE1, T10K17.80
Sequence domains: Ubiquitin-conjugating enzyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 32.924Å b: 50.382Å c: 93.776Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.216
Expression system: Escherichia coli

Quick links

Citations

3 review citations

SUMOylation in Phytopathogen Interactions: Balancing Invasion and Resistance.
Sharma et al. (2021)
2 more

1 mention without citation

The Structure of the Arabidopsis PEX4-PEX22 Peroxin Complex-Insights Into Ubiquitination at the Peroxisomal Membrane.
Traver et al. (2022)