› 6gvv

X-ray diffraction
2.35Å resolution

Mutant M16A of RNA dependent RNA polymerase 3D from Foot-and-Mouth disease Virus

Released:
DOI: 10.2210/pdb6gvv/pdb
PDB entry 6gvv coloured by chain, front view.
PDB entry 6gvv coloured by chain, side view.
PDB entry 6gvv coloured by chain, top view.
Source organism: Foot and mouth disease virus C
Primary publication:
Contribution of a Multifunctional Polymerase Region of Foot-and-Mouth Disease Virus to Lethal Mutagenesis.
de la Higuera I, Ferrer-Orta C, Moreno E, de Ávila AI, Soria ME, Singh K, Caridi F, Sobrino F, Sarafianos SG, Perales C, Verdaguer N, Domingo E
J Virol 92 (2018)
PMID: 30068642

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136761 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase 3D-POL Chain: A
Molecule details ›
Chain: A
Length: 481 amino acids
Theoretical weight: 54.05 KDa
Source organism: Foot and mouth disease virus C
Expression system: Escherichia coli
UniProt:
  • Canonical: P03311 (Residues: 1858-2327; Coverage: 20%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41212
Unit cell:
a: 93.12Å b: 93.12Å c: 121.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.263
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Potential molecular targets of nonstructural proteins for the development of antiviral drugs against SARS-CoV-2 infection.
Liu et al. (2021)
1 other article

1 mention without citation

Contribution of a Multifunctional Polymerase Region of Foot-and-Mouth Disease Virus to Lethal Mutagenesis.
de la Higuera et al. (2018)