6gwp

X-ray diffraction
2.28Å resolution

Crystal Structure of Stabilized Active Plasminogen Activator Inhibitor-1 (PAI-1-stab) in Complex with Two Inhibitory Nanobodies (VHH-2g-42, VHH-2w-64)

Released:
Model geometry
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Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-209235 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Plasminogen activator inhibitor 1 Chain: A
Molecule details ›
Chain: A
Length: 379 amino acids
Theoretical weight: 42.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05121 (Residues: 24-402; Coverage: 100%)
Gene names: PAI1, PLANH1, SERPINE1
Sequence domains: Serpin (serine protease inhibitor)
VHH-2g-42 Chain: B
Molecule details ›
Chain: B
Length: 116 amino acids
Theoretical weight: 12.75 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli BL21(DE3)
VHH-2w-64 Chain: C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21
Unit cell:
a: 43.681Å b: 70.797Å c: 98.513Å
α: 90° β: 97.48° γ: 90°
R-values:
R R work R free
0.204 0.203 0.225
Expression system: Escherichia coli BL21(DE3)