› 6gyg

X-ray diffraction
1.98Å resolution

X-ray structure of the apo form of the establishement gene regulator Reg576 of the G+ plasmid p576

Released:
DOI: 10.2210/pdb6gyg/pdb
PDB entry 6gyg coloured by chain, front view.
PDB entry 6gyg coloured by chain, side view.
PDB entry 6gyg coloured by chain, top view.
Source organism: Bacillus altitudinis
Primary publication:
Novel regulatory mechanism of establishment genes of conjugative plasmids.
Val-Calvo J, Luque-Ortega JR, Crespo I, Miguel-Arribas A, Abia D, Sánchez-Hevia DL, Serrano E, Gago-Córdoba C, Ares S, Alfonso C, Rojo F, Wu LJ, Boer DR, Meijer WJJ
Nucleic Acids Res 46 11910-11926 (2018)
PMID: 30380104

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166787 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription regulator Reg576 Chains: A, B
Molecule details ›
Chains: A, B
Length: 70 amino acids
Theoretical weight: 8.74 KDa
Source organism: Bacillus altitudinis
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P43212
Unit cell:
a: 44.278Å b: 44.278Å c: 141.166Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.207 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

The TraK accessory factor activates substrate transfer through the pKM101 type IV secretion system independently of its role in relaxosome assembly.
Li et al. (2020)
4 more

1 mention without citation

Novel regulatory mechanism of establishment genes of conjugative plasmids.
Val-Calvo et al. (2018)