6gys

Electron Microscopy
4.4Å resolution

Cryo-EM structure of the CBF3-CEN3 complex of the budding yeast kinetochore

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Architecture of the CBF3-centromere complex of the budding yeast kinetochore.
Nat Struct Mol Biol 25 1103-1110 (2018)
PMID: 30478265
Related structures: EMD-0096

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-119978 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
Centromere DNA-binding protein complex CBF3 subunit C Chains: A, H
Molecule details ›
Chains: A, H
Length: 478 amino acids
Theoretical weight: 56.42 KDa
Source organism: Saccharomyces cerevisiae
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P35203 (Residues: 1-478; Coverage: 100%)
Gene names: CBF3C, CTF13, YM6543.01, YM9582.19, YMR094W
Centromere DNA-binding protein complex CBF3 subunit B Chains: B, C, I, J
Molecule details ›
Chains: B, C, I, J
Length: 608 amino acids
Theoretical weight: 71.44 KDa
Source organism: Saccharomyces cerevisiae
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P40969 (Residues: 1-608; Coverage: 100%)
Gene names: CBF3, CBF3B, CEP3, CSL1, YM8520.17C, YMR168C
Sequence domains:
Suppressor of kinetochore protein 1 Chains: D, K
Molecule details ›
Chains: D, K
Length: 194 amino acids
Theoretical weight: 22.36 KDa
Source organism: Saccharomyces cerevisiae
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P52286 (Residues: 1-194; Coverage: 100%)
Gene names: CBF3D, D9798.14, SKP1, YDR328C
Sequence domains:
Centromere DNA-binding protein complex CBF3 subunit A Chains: E, L
Molecule details ›
Chains: E, L
Length: 956 amino acids
Theoretical weight: 112.07 KDa
Source organism: Saccharomyces cerevisiae
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P32504 (Residues: 1-956; Coverage: 100%)
Gene names: CBF2, CBF3A, CEP2, CTF14, NDC10, YGR140W
Sequence domains:
DNA (52-MER) Chain: F
Molecule details ›
Chain: F
Length: 52 nucleotides
Theoretical weight: 16.17 KDa
DNA (52-MER) Chain: G
Molecule details ›
Chain: G
Length: 52 nucleotides
Theoretical weight: 15.86 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-0096
Expression system: unidentified baculovirus