› 6gyy

X-ray diffraction
2.77Å resolution

Crystal structure of DacA from Staphylococcus aureus, N166C/T172C double mutant

Released:
DOI: 10.2210/pdb6gyy/pdb
PDB entry 6gyy coloured by chain, front view.
PDB entry 6gyy coloured by chain, side view.
PDB entry 6gyy coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Inhibition of the Staphylococcus aureus c-di-AMP cyclase DacA by direct interaction with the phosphoglucosamine mutase GlmM.
Tosi T, Hoshiga F, Millership C, Singh R, Eldrid C, Patin D, Mengin-Lecreulx D, Thalassinos K, Freemont P, Gründling A
PLoS Pathog 15 e1007537 (2019)
PMID: 30668586

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diadenylate cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 175 amino acids
Theoretical weight: 19.39 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
Structure domains: DNA integrity scanning protein, DisA, N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 44.862Å b: 79.773Å c: 98.812Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.231
Expression system: Escherichia coli

Quick links

Citations

7 review citations

A decade of research on the second messenger c-di-AMP.
Yin et al. (2020)
6 more

1 mention without citation

Inhibition of the Staphylococcus aureus c-di-AMP cyclase DacA by direct interaction with the phosphoglucosamine mutase GlmM.
Tosi et al. (2019)