› 6h02

X-ray diffraction
2.8Å resolution

Crystal structure of human Mediator subunit MED23

Released:
DOI: 10.2210/pdb6h02/pdb
PDB entry 6h02 coloured by chain, front view.
PDB entry 6h02 coloured by chain, side view.
PDB entry 6h02 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of human Mediator subunit MED23.
Monté D, Clantin B, Dewitte F, Lens Z, Rucktooa P, Pardon E, Steyaert J, Verger A, Villeret V
Nat Commun 9 3389 (2018)
PMID: 30140054

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212833 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mediator of RNA polymerase II transcription subunit 23 Chain: A
Molecule details ›
Chain: A
Length: 1382 amino acids
Theoretical weight: 158.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9ULK4 (Residues: 1-1368; Coverage: 100%)
Gene names: ARC130, CRSP3, DRIP130, KIAA1216, MED23, SUR2
Sequence domains: Mediator complex subunit 23
LAMA nanobody NB106 Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 14.75 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21
Unit cell:
a: 75.65Å b: 137.18Å c: 90.11Å
α: 90° β: 110.98° γ: 90°
R-values:
R R work R free
0.201 0.199 0.24
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 review citation

The Structures of Eukaryotic Transcription Pre-initiation Complexes and Their Functional Implications.
Greber et al. (2019)
4 other articles

5 mentions without citation

Twenty years of Mediator complex structural studies.
Verger et al. (2019)
4 more