› 6h07

X-ray diffraction
1.48Å resolution

X-ray structure of Lactobacillus brevis alcohol dehydrogenase

Released:
DOI: 10.2210/pdb6h07/pdb
PDB entry 6h07 coloured by chain, front view.
PDB entry 6h07 coloured by chain, side view.
PDB entry 6h07 coloured by chain, top view.
Source organism: Levilactobacillus brevis
Primary publication:
Neutron and X-ray crystal structures of Lactobacillus brevis alcohol dehydrogenase reveal new insights into hydrogen-bonding pathways.
Hermann J, Nowotny P, Schrader TE, Biggel P, Hekmat D, Weuster-Botz D
Acta Crystallogr F Struct Biol Commun 74 754-764 (2018)
PMID: 30511668

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
R-specific alcohol dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 26.66 KDa
Source organism: Levilactobacillus brevis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q84EX5 (Residues: 2-252; Coverage: 100%)
Gene name: radh
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Unit cell:
a: 56.03Å b: 83.31Å c: 114.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.203
Expression system: Escherichia coli BL21(DE3)

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Citations

2 mentions without citation

Deracemisation and stereoinversion by a nanoconfined bidirectional enzyme cascade: dual control by electrochemistry and selective metal ion activation.
Cheng et al. (2022)
1 more