› 6h1d

X-ray diffraction
1.94Å resolution

Crystal structure of C21orf127-TRMT112 in complex with SAH

Released:
DOI: 10.2210/pdb6h1d/pdb
PDB entry 6h1d coloured by chain, front view.
PDB entry 6h1d coloured by chain, side view.
PDB entry 6h1d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
KMT9 monomethylates histone H4 lysine 12 and controls proliferation of prostate cancer cells.
Metzger E, Wang S, Urban S, Willmann D, Schmidt A, Offermann A, Allen A, Sum M, Obier N, Cottard F, Ulferts S, Preca BT, Hermann B, Maurer J, Greschik H, Hornung V, Einsle O, Perner S, Imhof A, Jung M, Schüle R
Nat Struct Mol Biol 26 361-371 (2019)
PMID: 31061526

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193774 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methyltransferase N6AMT1 Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 22.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5N5 (Residues: 8-214; Coverage: 97%)
Gene names: C21orf127, HEMK2, KMT9, N6AMT1, PRED28
Sequence domains: Methyltransferase small domain
Multifunctional methyltransferase subunit TRM112-like protein Chain: B
Molecule details ›
Chain: B
Length: 126 amino acids
Theoretical weight: 14.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI30 (Residues: 2-125; Coverage: 99%)
Gene names: AD-001, HSPC152, HSPC170, TRMT112
Sequence domains: Trm112p-like protein

Ligands and Environments

1 bound ligand:
Ligand SAH 1 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P42212
Unit cell:
a: 71.841Å b: 71.841Å c: 131.113Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.237
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Histone lysine methyltransferases in biology and disease.
Husmann et al. (2019)
9 more

2 mentions without citation

Human TRMT112-Methyltransferase Network Consists of Seven Partners Interacting with a Common Co-Factor.
Brūmele et al. (2021)
1 more