› 6h2v

X-ray diffraction
2.49Å resolution

Crystal structure of human METTL5-TRMT112 complex, the 18S rRNA m6A1832 methyltransferase at 2.5A resolution

Released:
DOI: 10.2210/pdb6h2v/pdb
PDB entry 6h2v coloured by chain, front view.
PDB entry 6h2v coloured by chain, side view.
PDB entry 6h2v coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The human 18S rRNA m6A methyltransferase METTL5 is stabilized by TRMT112.
van Tran N, Ernst FGM, Hawley BR, Zorbas C, Ulryck N, Hackert P, Bohnsack KE, Bohnsack MT, Jaffrey SR, Graille M, Lafontaine DLJ
Nucleic Acids Res 47 7719-7733 (2019)
PMID: 31328227

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192566 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
rRNA N6-adenosine-methyltransferase METTL5 Chains: A, C
Molecule details ›
Chains: A, C
Length: 215 amino acids
Theoretical weight: 24.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NRN9 (Residues: 1-209; Coverage: 100%)
Gene names: DC3, HSPC133, METTL5
Sequence domains: Methyltransferase small domain
Multifunctional methyltransferase subunit TRM112-like protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 125 amino acids
Theoretical weight: 14.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI30 (Residues: 1-125; Coverage: 100%)
Gene names: AD-001, HSPC152, HSPC170, TRMT112
Sequence domains: Trm112p-like protein

Ligands and Environments

5 bound ligands:
Ligand SAM 2 x SAM
Ligand SO4 7 x SO4
Ligand PEG 1 x PEG
Ligand EDO 2 x EDO
Ligand PG0 2 x PG0
1 modified residue:
Ligand CME 2 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P212121
Unit cell:
a: 56.38Å b: 105.894Å c: 169.239Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.249
Expression system: Escherichia coli

Quick links

Citations

137 review citations

m6A Modification in Coding and Non-coding RNAs: Roles and Therapeutic Implications in Cancer.
Huang et al. (2020)
136 more

7 mentions without citation

A comprehensive review of m6A/m6Am RNA methyltransferase structures.
Oerum et al. (2021)
6 more