› 6h3l

Electron Microscopy
4.2Å resolution

Structure of VgrG1 in the Type VI secretion "pre-firing" VgrG1-Tse6-EagT6-EF-Tu-Tsi6 complex

Released:
DOI: 10.2210/pdb6h3l/pdb
PDB entry 6h3l coloured by chain, front view.
PDB entry 6h3l coloured by chain, side view.
PDB entry 6h3l coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Mechanism of loading and translocation of type VI secretion system effector Tse6.
Quentin D, Ahmad S, Shanthamoorthy P, Mougous JD, Whitney JC, Raunser S
Nat Microbiol 3 1142-1152 (2018)
PMID: 30177742
Related structures: EMD-0135

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type VI secretion system spike protein VgrG1a Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 643 amino acids
Theoretical weight: 72.1 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9I741 (Residues: 1-643; Coverage: 100%)
Gene names: PA0091, vgrG1a
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-0135
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Polymorphic Toxins and Their Immunity Proteins: Diversity, Evolution, and Mechanisms of Delivery.
Ruhe et al. (2020)
3 more

5 mentions without citation

Mechanism of loading and translocation of type VI secretion system effector Tse6.
Quentin et al. (2018)
4 more