› 6h49

X-ray diffraction
1.8Å resolution

A polyamorous repressor: deciphering the evolutionary strategy used by the phage-inducible chromosomal islands to spread in nature.

Released:
DOI: 10.2210/pdb6h49/pdb
PDB entry 6h49 coloured by chain, front view.
PDB entry 6h49 coloured by chain, side view.
PDB entry 6h49 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature.
Ciges-Tomas JR, Alite C, Humphrey S, Donderis J, Bowring J, Salvatella X, Penadés JR, Marina A
Nat Commun 10 3676 (2019)
PMID: 31417084

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190232 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH cro/C1-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 18.16 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9F0J8 (Residues: 1-156; Coverage: 58%)
Sequence domains: Helix-turn-helix

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 132.538Å b: 34.553Å c: 36.99Å
α: 90° β: 95.96° γ: 90°
R-values:
R R work R free
0.174 0.172 0.216
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

A regulatory cascade controls Staphylococcus aureus pathogenicity island activation.
Haag et al. (2021)
3 more

3 mentions without citation

Non-canonical Staphylococcus aureus pathogenicity island repression.
Miguel-Romero et al. (2022)
2 more