› 6h4j

X-ray diffraction
3.07Å resolution

Usp25 catalytic domain

Released:
DOI: 10.2210/pdb6h4j/pdb
PDB entry 6h4j coloured by chain, front view.
PDB entry 6h4j coloured by chain, side view.
PDB entry 6h4j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Differential Oligomerization of the Deubiquitinases USP25 and USP28 Regulates Their Activities.
Sauer F, Klemm T, Kollampally RB, Tessmer I, Nair RK, Popov N, Kisker C
Mol Cell 74 421-435.e10 (2019)
PMID: 30926243

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 25 Chains: A, B
Molecule details ›
Chains: A, B
Length: 552 amino acids
Theoretical weight: 64.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UHP3 (Residues: 157-706; Coverage: 52%)
Gene names: USP21, USP25
Sequence domains: Ubiquitin carboxyl-terminal hydrolase

Ligands and Environments

1 bound ligand:
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2221
Unit cell:
a: 81.945Å b: 202.629Å c: 169.594Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Deubiquitylases in developmental ubiquitin signaling and congenital diseases.
Basar et al. (2021)
7 more