6h4k

X-ray diffraction
2.05Å resolution

Structure of the Usp25 C-terminal domain

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-193758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 25 Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 35.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UHP3 (Residues: 765-1055; Coverage: 28%)
Gene names: USP21, USP25

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P43212
Unit cell:
a: 87.008Å b: 87.008Å c: 86.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.222
Expression system: Escherichia coli BL21(DE3)