› 6h5h

Solution NMR

A computationally designed dRP lyase domain reconstructed from two heterologous fragments

Released:
DOI: 10.2210/pdb6h5h/pdb
PDB entry 6h5h coloured by chain, ensemble of 22 models, front view.
PDB entry 6h5h coloured by chain, ensemble of 22 models, side view.
PDB entry 6h5h coloured by chain, ensemble of 22 models, top view.
Source organism: synthetic construct
Primary publication:
Asymmetric protein design from conserved supersecondary structures.
ElGamacy M, Coles M, Lupas A
J Struct Biol 204 380-387 (2018)
PMID: 30558718

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
polb4 Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 13.44 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: DNA polymerase beta, N-terminal domain-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 81%
Refinement method: NOESY back-calculation
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Evolution, folding, and design of TIM barrels and related proteins.
Romero-Romero et al. (2021)
4 other articles

1 mention without citation

De Novo Protein Design for Novel Folds Using Guided Conditional Wasserstein Generative Adversarial Networks.
Karimi et al. (2020)