› 6h7e

X-ray diffraction
2.3Å resolution

GEF regulatory domain

Released:
DOI: 10.2210/pdb6h7e/pdb
PDB entry 6h7e coloured by chain, front view.
PDB entry 6h7e coloured by chain, side view.
PDB entry 6h7e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Membranes prime the RapGEF EPAC1 to transduce cAMP signaling.
Sartre C, Peurois F, Ley M, Kryszke MH, Zhang W, Courilleau D, Fischmeister R, Ambroise Y, Zeghouf M, Cianferani S, Ferrandez Y, Cherfils J
Nat Commun 14 4157 (2023)
PMID: 37438343

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131916 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rap guanine nucleotide exchange factor 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 270 amino acids
Theoretical weight: 30.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: O95398 (Residues: 92-360; Coverage: 29%)
Gene names: CGEF1, EPAC, EPAC1, RAPGEF3
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CMP 2 x CMP
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Unit cell:
a: 41.698Å b: 105.617Å c: 160.556Å
α: 90° β: 90.04° γ: 90°
R-values:
R R work R free
0.197 0.195 0.237
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

Rap1 in the Context of PCSK9, Atherosclerosis, and Diabetes.
Agarwal et al. (2023)