› 6hay

X-ray diffraction
2.24Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-154076 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Probable global transcription activator SNF2L2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 123 amino acids
Theoretical weight: 14.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51531 (Residues: 1373-1511; Coverage: 8%)
Gene names: BAF190B, BRM, SMARCA2, SNF2A, SNF2L2
Sequence domains: Bromodomain
von Hippel-Lindau disease tumor suppressor Chains: B, F
Molecule details ›
Chains: B, F
Length: 162 amino acids
Theoretical weight: 18.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40337 (Residues: 54-213; Coverage: 75%)
Gene name: VHL
Sequence domains:
Elongin-C Chains: C, G
Molecule details ›
Chains: C, G
Length: 97 amino acids
Theoretical weight: 10.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Elongin-B Chains: D, H
Molecule details ›
Chains: D, H
Length: 104 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15370 (Residues: 1-104; Coverage: 88%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family

Ligands and Environments

4 bound ligands:
Ligand FX8 2 x FX8
Ligand EDO 22 x EDO
Ligand FMT 1 x FMT
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 79.725Å b: 117.26Å c: 121.557Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

68 review citations

PROTAC targeted protein degraders: the past is prologue.
Békés et al. (2022)
67 more

15 mentions without citation

Advancing targeted protein degradation for cancer therapy.
Dale et al. (2021)
14 more