› 6hck

X-ray diffraction
2.7Å resolution

The Transcriptional Regulator PrfA from Listeria Monocytogenes in complex with dipeptide Leu-Leu

Released:
DOI: 10.2210/pdb6hck/pdb
PDB entry 6hck coloured by chain, front view.
PDB entry 6hck coloured by chain, side view.
PDB entry 6hck coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Primary publication:
Control of Bacterial Virulence through the Peptide Signature of the Habitat.
Krypotou E, Scortti M, Grundström C, Oelker M, Luisi BF, Sauer-Eriksson AE, Vázquez-Boland J
Cell Rep 26 1815-1827.e5 (2019)
PMID: 30759392

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Listeriolysin regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27.46 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli
UniProt:
  • Canonical: P22262 (Residues: 1-237; Coverage: 100%)
Gene names: lmo0200, prfA
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand LEU 2 x LEU
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 47.948Å b: 87.394Å c: 116.883Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.209 0.27
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Cross Talk between SigB and PrfA in Listeria monocytogenes Facilitates Transitions between Extra- and Intracellular Environments.
Gaballa et al. (2019)
4 more

1 mention without citation

Control of Bacterial Virulence through the Peptide Signature of the Habitat.
Krypotou et al. (2019)