› 6hco

Electron Microscopy
3.58Å resolution

Cryo-EM structure of the ABCG2 E211Q mutant bound to estrone 3-sulfate and 5D3-Fab

Released:
DOI: 10.2210/pdb6hco/pdb
PDB entry 6hco coloured by chain, front view.
PDB entry 6hco coloured by chain, side view.
PDB entry 6hco coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures of a human ABCG2 mutant trapped in ATP-bound and substrate-bound states.
Manolaridis I, Jackson SM, Taylor NMI, Kowal J, Stahlberg H, Locher KP
Nature 563 426-430 (2018)
PMID: 30405239
Related structures: EMD-0196

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212845 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Broad substrate specificity ATP-binding cassette transporter ABCG2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 664 amino acids
Theoretical weight: 73.39 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UNQ0 (Residues: 2-655; Coverage: 100%)
Gene names: ABCG2, ABCP, BCRP, BCRP1, MXR
Sequence domains:
5D3-Fab light chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 214 amino acids
Theoretical weight: 23.59 KDa
Source organism: Mus musculus
Expression system: Mus musculus
5D3-Fab heavy chain Chains: D, F
Molecule details ›
Chains: D, F
Length: 221 amino acids
Theoretical weight: 23.84 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand FY5 1 x FY5
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.58Å
Relevant EMDB volumes: EMD-0196
Expression systems:
  • Homo sapiens
  • Mus musculus

Quick links

Citations

16 review citations

Structures of ABC transporters: handle with care.
Lewinson et al. (2020)
15 more

22 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
21 more