› 6hel

X-ray diffraction
2.94Å resolution

Structure of human USP25

Released:
DOI: 10.2210/pdb6hel/pdb
PDB entry 6hel coloured by chain, front view.
PDB entry 6hel coloured by chain, side view.
PDB entry 6hel coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Distinct USP25 and USP28 Oligomerization States Regulate Deubiquitinating Activity.
Gersch M, Wagstaff JL, Toms AV, Graves B, Freund SMV, Komander D
Mol Cell 74 436-451.e7 (2019)
PMID: 30926242

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 25 Chains: A, B
Molecule details ›
Chains: A, B
Length: 560 amino acids
Theoretical weight: 65.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UHP3 (Residues: 157-714; Coverage: 53%)
Gene names: USP21, USP25
Sequence domains: Ubiquitin carboxyl-terminal hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I4
Unit cell:
a: 139.184Å b: 139.184Å c: 190.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.254 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Deubiquitylases in developmental ubiquitin signaling and congenital diseases.
Basar et al. (2021)
10 more

2 mentions without citation

Molecular Mechanisms of DUBs Regulation in Signaling and Disease.
Li et al. (2021)
1 more