› 6hik

X-ray diffraction
1.65Å resolution

X-ray structure of TEAD4(Y429H) mutant) complexed with YAP (wildtype): Molecular and structural characterization of a TEAD mutation at the origin of Sveinsson's chorioretinal atrophy

Released:
DOI: 10.2210/pdb6hik/pdb
PDB entry 6hik coloured by chain, front view.
PDB entry 6hik coloured by chain, side view.
PDB entry 6hik coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular and structural characterization of a TEAD mutation at the origin of Sveinsson's chorioretinal atrophy.
Bokhovchuk F, Mesrouze Y, Izaac A, Meyerhofer M, Zimmermann C, Fontana P, Schmelzle T, Erdmann D, Furet P, Kallen J, Chène P
FEBS J 286 2381-2398 (2019)
PMID: 30903741

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155554 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcriptional enhancer factor TEF-3 Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 25.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15561 (Residues: 216-434; Coverage: 51%)
Gene names: RTEF1, TCF13L1, TEAD4, TEF3
Sequence domains: YAP binding domain
Structure domains: Coagulation Factor XIII; Chain A, domain 1
Transcriptional coactivator YAP1 Chain: L
Molecule details ›
Chain: L
Length: 41 amino acids
Theoretical weight: 4.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P46937 (Residues: 60-99; Coverage: 8%)
Gene names: YAP1, YAP65
Structure domains: Virus Scaffolding Protein; Chain A

Ligands and Environments

2 bound ligands:
Ligand MYR 1 x MYR
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41212
Unit cell:
a: 58.843Å b: 58.843Å c: 159.101Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.237
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Regulation of TEAD Transcription Factors in Cancer Biology.
Huh et al. (2019)
5 more

1 mention without citation

Molecular and structural characterization of a TEAD mutation at the origin of Sveinsson's chorioretinal atrophy.
Bokhovchuk et al. (2019)