› 6hip

X-ray diffraction
1.2Å resolution

Structure of SPF45 UHM bound to HIV-1 Rev ULM

Released:
DOI: 10.2210/pdb6hip/pdb
PDB entry 6hip coloured by chain, front view.
PDB entry 6hip coloured by chain, side view.
PDB entry 6hip coloured by chain, top view.
Source organisms:
Primary publication:
Modulation of HIV-1 gene expression by binding of a ULM motif in the Rev protein to UHM-containing splicing factors.
Pabis M, Corsini L, Vincendeau M, Tripsianes K, Gibson TJ, Brack-Werner R, Sattler M
Nucleic Acids Res 47 4859-4871 (2019)
PMID: 30892606

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-138057 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Splicing factor 45 Chains: A, B
Molecule details ›
Chains: A, B
Length: 104 amino acids
Theoretical weight: 11.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96I25 (Residues: 301-401; Coverage: 25%)
Gene names: RBM17, SPF45
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Protein Rev Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.4 KDa
Source organism: Human immunodeficiency virus 1
Expression system: synthetic construct
UniProt:
  • Canonical: P04618 (Residues: 41-49; Coverage: 8%)
Gene name: rev

Ligands and Environments

2 bound ligands:
Ligand TRP 1 x TRP
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 48.21Å b: 63.68Å c: 67.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.175
Expression systems:
  • Escherichia coli
  • synthetic construct

Quick links

Citations

1 review citation

HIV-1: To Splice or Not to Splice, That Is the Question.
Emery et al. (2021)
3 other articles

1 mention without citation

Modulation of HIV-1 gene expression by binding of a ULM motif in the Rev protein to UHM-containing splicing factors.
Pabis et al. (2019)