› 6hiz

Electron Microscopy
3.08Å resolution

Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the head of the small mitoribosomal subunit

Released:
DOI: 10.2210/pdb6hiz/pdb
PDB entry 6hiz coloured by chain, front view.
PDB entry 6hiz coloured by chain, side view.
PDB entry 6hiz coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes.
Ramrath DJF, Niemann M, Leibundgut M, Bieri P, Prange C, Horn EK, Leitner A, Boehringer D, Schneider A, Ban N
Science 362 (2018)
PMID: 30213880
Related structures: EMD-0233

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
PDBe Complex ID:
PDB-CPX-302917 (preferred)
Entry contents:
27 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (28 distinct):
mS48 Chain: DA
Molecule details ›
Chain: DA
Length: 1788 amino acids
Theoretical weight: 201.81 KDa
Source organism: Trypanosoma brucei brucei
UniProt:
  • Canonical: Q57UJ2 (Residues: 1-1788; Coverage: 100%)
Gene names: Tb08.5H5.480, Tb927.8.5200
mS59 Chain: DL
Molecule details ›
Chain: DL
Length: 307 amino acids
Theoretical weight: 35.4 KDa
Source organism: Trypanosoma brucei brucei
UniProt:
  • Canonical: Q38BS2 (Residues: 1-307; Coverage: 100%)
Gene name: Tb10.70.4220
mS49 Chain: DB
mS50 Chain: DC
Mitochondrial SSU ribosomal protein Chain: DE
mS53 Chain: DF
mS54 Chain: DG
Pentacotripeptide-repeat region of PRORP domain-containing protein Chain: DH
mS57 Chain: DJ
mS58 Chain: DK
Rhodanese domain-containing protein Chain: DT
mS69 Chain: DV
mS70 Chain: DW
mS71 Chain: DX
mS72 Chain: DY
uS3m Chain: CC
Ribosomal protein S9 Chain: CI
LysM domain-containing protein Chain: CJ
uS11m Chain: CK
uS14m Chain: CN
Mitochondrial ribosomal protein S18 Chain: CR
uS19m Chain: CS
Small ribosomal subunit protein mS29 Chain: Cg
mS33 Chain: Ci
Mitochondrial SSU ribosomal protein Chain: Ck
Unknown protein Chain: UO
Unknown protein Chain: UP
RNA (143-MER) Chain: CA

Ligands and Environments

4 bound ligands:
Ligand SPD 2 x SPD
Ligand UTP 1 x UTP
Ligand GTP 1 x GTP
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.08Å
Relevant EMDB volumes: EMD-0233

Quick links

Citations

15 review citations

Mechanisms and regulation of protein synthesis in mitochondria.
Kummer et al. (2021)
14 more

1 mention without citation

Measurement of atom resolvability in cryo-EM maps with Q-scores.
Pintilie et al. (2020)