› 6hpu

X-ray diffraction
3.96Å resolution

Crystal structure of human Pif1 helicase in complex with ADP-AlF4

Released:
DOI: 10.2210/pdb6hpu/pdb
PDB entry 6hpu coloured by chain, front view.
PDB entry 6hpu coloured by chain, side view.
PDB entry 6hpu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of the nucleotide and DNA binding activities of the human PIF1 helicase.
Dehghani-Tafti S, Levdikov V, Antson AA, Bax B, Sanders CM
Nucleic Acids Res 47 3208-3222 (2019)
PMID: 30698796

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190630 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent DNA helicase PIF1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 418 amino acids
Theoretical weight: 45.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H611 (Residues: 206-620; Coverage: 65%)
Gene names: C15orf20, PIF1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand ALF 2 x ALF
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3221
Unit cell:
a: 209.85Å b: 209.85Å c: 78.885Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.175 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

History of DNA Helicases.
Brosh et al. (2020)
5 more

3 mentions without citation

Structural and functional analysis of the nucleotide and DNA binding activities of the human PIF1 helicase.
Dehghani-Tafti et al. (2019)
2 more