› 6hra

Electron Microscopy
3.7Å resolution

Cryo-EM structure of the KdpFABC complex in an E1 outward-facing state (state 1)

Released:
DOI: 10.2210/pdb6hra/pdb
PDB entry 6hra coloured by chain, front view.
PDB entry 6hra coloured by chain, side view.
PDB entry 6hra coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Cryo-EM structures of KdpFABC suggest a K+ transport mechanism via two inter-subunit half-channels.
Stock C, Hielkema L, Tascón I, Wunnicke D, Oostergetel GT, Azkargorta M, Paulino C, Hänelt I
Nat Commun 9 4971 (2018)
PMID: 30478378
Related structures: EMD-0257

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + K(+)(Side 1) = ADP + phosphate + K(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137301 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Potassium-transporting ATPase potassium-binding subunit Chain: A
Molecule details ›
Chain: A
Length: 557 amino acids
Theoretical weight: 59.22 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03959 (Residues: 1-557; Coverage: 100%)
Gene names: JW0686, b0698, kdpA
Sequence domains: Potassium-transporting ATPase A subunit
Potassium-transporting ATPase KdpC subunit Chain: C
Molecule details ›
Chain: C
Length: 190 amino acids
Theoretical weight: 20.28 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03961 (Residues: 1-190; Coverage: 100%)
Gene names: JW0684, b0696, kdpC
Sequence domains: K+-transporting ATPase, c chain
Potassium-transporting ATPase KdpF subunit Chain: D
Molecule details ›
Chain: D
Length: 29 amino acids
Theoretical weight: 3.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P36937 (Residues: 1-29; Coverage: 100%)
Gene names: JW0687, b4513, kdpF
Sequence domains: F subunit of K+-transporting ATPase (Potass_KdpF)
Potassium-transporting ATPase ATP-binding subunit Chain: B
Molecule details ›
Chain: B
Length: 682 amino acids
Theoretical weight: 72.35 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03960 (Residues: 1-682; Coverage: 100%)
Gene names: JW0685, b0697, kdpB
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand K 3 x K
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-0257
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Bacterial Small Membrane Proteins: the Swiss Army Knife of Regulators at the Lipid Bilayer.
Yadavalli et al. (2022)
5 more

6 mentions without citation

Molecular Mechanisms for Bacterial Potassium Homeostasis.
Stautz et al. (2021)
5 more