› 6hsn

X-ray diffraction
1.55Å resolution

Crystal structure of the ternary complex of GephE-ADP-GABA(A) receptor derived peptide

Released:
DOI: 10.2210/pdb6hsn/pdb
PDB entry 6hsn coloured by chain, front view.
PDB entry 6hsn coloured by chain, side view.
PDB entry 6hsn coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Elucidating the Molecular Basis for Inhibitory Neurotransmission Regulation by Artemisinins.
Kasaragod VB, Hausrat TJ, Schaefer N, Kuhn M, Christensen NR, Tessmer I, Maric HM, Madsen KL, Sotriffer C, Villmann C, Kneussel M, Schindelin H
Neuron 101 673-689.e11 (2019)
PMID: 30704910

Function and Biology Details

Reactions catalysed: 
Adenylyl-molybdopterin + molybdate = molybdenum cofactor + AMP
ATP + molybdopterin = diphosphate + adenylyl-molybdopterin
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148881 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gephyrin Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 45.65 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03555 (Residues: 350-768; Coverage: 55%)
Gene names: Gph, Gphn
Sequence domains:
Structure domains:
Gamma-aminobutyric acid receptor subunit alpha-3 Chains: D, E
Molecule details ›
Chains: D, E
Length: 10 amino acids
Theoretical weight: 1.11 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P20236 (Residues: 396-405; Coverage: 2%)
Gene names: Gabra-3, Gabra3

Ligands and Environments

7 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 2 x MG
Ligand MPD 3 x MPD
Ligand ACT 8 x ACT
Ligand PO4 1 x PO4
Ligand NA 1 x NA
Ligand 3F8 1 x 3F8
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: I222
Unit cell:
a: 86.962Å b: 99.217Å c: 111.608Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.196
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Looking for Novelty in an "Old" Receptor: Recent Advances Toward Our Understanding of GABAARs and Their Implications in Receptor Pharmacology.
Castellano et al. (2020)
11 other articles