› 6hsw

X-ray diffraction
2.15Å resolution

A CE15 glucuronoyl esterase from Teredinibacter turnerae T7901

Released:
DOI: 10.2210/pdb6hsw/pdb
PDB entry 6hsw coloured by chain, front view.
PDB entry 6hsw coloured by chain, side view.
PDB entry 6hsw coloured by chain, top view.
Source organism: Teredinibacter turnerae T7901
Primary publication:
Structure-function analyses reveal that a glucuronoyl esterase from Teredinibacter turnerae interacts with carbohydrates and aromatic compounds.
Arnling Bååth J, Mazurkewich S, Poulsen JN, Olsson L, Lo Leggio L, Larsbrink J
J Biol Chem 294 6635-6644 (2019)
PMID: 30814248

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111464 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate esterase family 15 domain protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 440 amino acids
Theoretical weight: 48.08 KDa
Source organism: Teredinibacter turnerae T7901
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5BN23 (Residues: 26-445; Coverage: 98%)
Gene name: TERTU_0517

Ligands and Environments

5 bound ligands:
Ligand 1PE 2 x 1PE
Ligand EDO 20 x EDO
Ligand GOL 13 x GOL
Ligand BR 2 x BR
Ligand PEG 1 x PEG
1 modified residue:
Ligand MSE 21 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P3121
Unit cell:
a: 121.173Å b: 121.173Å c: 198.203Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.164 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

New insights to diversity and enzyme-substrate interactions of fungal glucuronoyl esterases.
Agger et al. (2023)
1 more

6 mentions without citation

Glucuronoyl esterases - enzymes to decouple lignin and carbohydrates and enable better utilization of renewable plant biomass.
Larsbrink et al. (2023)
5 more