› 6hum

Electron Microscopy
3.34Å resolution

Structure of the photosynthetic complex I from Thermosynechococcus elongatus

Released:
DOI: 10.2210/pdb6hum/pdb
PDB entry 6hum coloured by chain, front view.
PDB entry 6hum coloured by chain, side view.
PDB entry 6hum coloured by chain, top view.
Source organism: Thermosynechococcus vestitus BP-1
Primary publication:
Structural adaptations of photosynthetic complex I enable ferredoxin-dependent electron transfer.
Schuller JM, Birrell JA, Tanaka H, Konuma T, Wulfhorst H, Cox N, Schuller SK, Thiemann J, Lubitz W, Sétif P, Ikegami T, Engel BD, Kurisu G, Nowaczyk MM
Science 363 257-260 (2019)
PMID: 30573545
Related structures: EMD-0281

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
PDBe Complex ID:
PDB-CPX-166835 (preferred)
Entry contents:
18 distinct polypeptide molecules
Macromolecules (18 distinct):
NAD(P)H-quinone oxidoreductase subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 372 amino acids
Theoretical weight: 40.57 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DL32 (Residues: 1-372; Coverage: 100%)
Gene names: ndhA, tlr0667
Sequence domains: NADH dehydrogenase
NAD(P)H-quinone oxidoreductase subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 132 amino acids
Theoretical weight: 15.01 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DJ02 (Residues: 1-132; Coverage: 100%)
Gene names: ndhC, tlr1429
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
Structure domains: NADH:ubiquinone/plastoquinone oxidoreductase, chain 3
NAD(P)H-quinone oxidoreductase chain 4 1 Chain: D
Molecule details ›
Chain: D
Length: 529 amino acids
Theoretical weight: 57.85 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DKY0 (Residues: 1-529; Coverage: 100%)
Gene names: ndhD1, tll0719
Sequence domains: Proton-conducting membrane transporter
NAD(P)H-quinone oxidoreductase subunit 4L Chain: E
Molecule details ›
Chain: E
Length: 101 amino acids
Theoretical weight: 11.14 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DL29 (Residues: 1-101; Coverage: 100%)
Gene name: ndhE
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
Structure domains: Helix Hairpins
NAD(P)H-quinone oxidoreductase subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 515 amino acids
Theoretical weight: 55.17 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DMR6 (Residues: 1-515; Coverage: 100%)
Gene names: ndhB, tll0045
Sequence domains:
NADH-quinone oxidoreductase subunit J Chain: G
Molecule details ›
Chain: G
Length: 200 amino acids
Theoretical weight: 21.58 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DL30 (Residues: 1-200; Coverage: 100%)
Gene name: ndhG
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 6
NAD(P)H-quinone oxidoreductase subunit J Chain: J
Molecule details ›
Chain: J
Length: 168 amino acids
Theoretical weight: 19.36 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DJ01 (Residues: 1-168; Coverage: 100%)
Gene names: ndhJ, tlr1430
Sequence domains: Respiratory-chain NADH dehydrogenase, 30 Kd subunit
Proton-translocating NADH-quinone dehydrogenase subunit P NdhP Chain: P
Molecule details ›
Chain: P
Length: 42 amino acids
Theoretical weight: 4.63 KDa
Source organism: Thermosynechococcus vestitus BP-1
NAD(P)H-quinone oxidoreductase subunit H Chain: H
Molecule details ›
Chain: H
Length: 394 amino acids
Theoretical weight: 45.27 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DJD9 (Residues: 1-394; Coverage: 100%)
Gene names: ndhH, tlr1288
Sequence domains: Respiratory-chain NADH dehydrogenase, 49 Kd subunit
NAD(P)H-quinone oxidoreductase subunit I Chain: I
Molecule details ›
Chain: I
Length: 196 amino acids
Theoretical weight: 22.44 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DL31 (Residues: 1-196; Coverage: 100%)
Gene names: ndhI, tlr0668
Sequence domains: 4Fe-4S dicluster domain
NAD(P)H-quinone oxidoreductase subunit K Chain: K
Molecule details ›
Chain: K
Length: 237 amino acids
Theoretical weight: 25.77 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DKZ4 (Residues: 1-237; Coverage: 100%)
Gene names: ndhK, tlr0705
Sequence domains: NADH ubiquinone oxidoreductase, 20 Kd subunit
NAD(P)H-quinone oxidoreductase subunit L Chain: L
Molecule details ›
Chain: L
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DKZ3 (Residues: 1-76; Coverage: 100%)
Gene names: ndhL, tsr0706
Sequence domains: NADH dehydrogenase transmembrane subunit
NADH dehydrogenase subunit 5 Chain: F
Molecule details ›
Chain: F
Length: 656 amino acids
Theoretical weight: 72.03 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DKX9 (Residues: 1-656; Coverage: 100%)
Gene name: ndhF1
Sequence domains:
NAD(P)H-quinone oxidoreductase subunit N Chain: N
Molecule details ›
Chain: N
Length: 150 amino acids
Theoretical weight: 16.66 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DJU2 (Residues: 1-150; Coverage: 100%)
Gene names: ndhN, tlr1130
Sequence domains: NADH-quinone oxidoreductase cyanobacterial subunit N
NAD(P)H-quinone oxidoreductase subunit M Chain: M
Molecule details ›
Chain: M
Length: 111 amino acids
Theoretical weight: 12.58 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DLN5 (Residues: 1-111; Coverage: 100%)
Gene names: ndhM, tll0447
Sequence domains: Cyanobacterial and plastid NDH-1 subunit M
DUF3252 domain-containing protein Chain: S
Molecule details ›
Chain: S
Length: 110 amino acids
Theoretical weight: 12.46 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DL61 (Residues: 1-110; Coverage: 100%)
Gene name: tlr0636
Sequence domains: NAD(P)H dehydrogenase subunit S
Structure domains: SH3 type barrels.
NAD(P)H-quinone oxidoreductase subunit O Chain: O
Molecule details ›
Chain: O
Length: 70 amino acids
Theoretical weight: 7.88 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: Q8DMU4 (Residues: 1-70; Coverage: 100%)
Gene names: ndhO, tsl0017
Sequence domains: Cyanobacterial and plant NDH-1 subunit O
Proton-translocating NADH-quinone dehydrogenase subunit Q NdhQ Chain: Q
Molecule details ›
Chain: Q
Length: 39 amino acids
Theoretical weight: 4.21 KDa
Source organism: Thermosynechococcus vestitus BP-1

Ligands and Environments

3 bound ligands:
Ligand BCR 1 x BCR
Ligand LMG 1 x LMG
Ligand SF4 3 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.34Å
Relevant EMDB volumes: EMD-0281

Quick links

Citations

13 review citations

Structure of respiratory complex I - An emerging blueprint for the mechanism.
Kampjut et al. (2022)
12 more

10 mentions without citation

Evolution of complex I-like respiratory complexes.
Yu et al. (2021)
9 more