› 6hvo

X-ray diffraction
2.1Å resolution

Crystal structure of human PCNA in complex with three peptides of p12 subunit of human polymerase delta

Released:
DOI: 10.2210/pdb6hvo/pdb
PDB entry 6hvo coloured by chain, front view.
PDB entry 6hvo coloured by chain, side view.
PDB entry 6hvo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The p12 subunit of human polymerase δ uses an atypical PIP box for molecular recognition of proliferating cell nuclear antigen (PCNA).
Gonzalez-Magaña A, Ibáñez de Opakua A, Romano-Moreno M, Murciano-Calles J, Merino N, Luque I, Rojas AL, Onesti S, Blanco FJ, De Biasio A
J Biol Chem 294 3947-3956 (2019)
PMID: 30655288

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146066 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 264 amino acids
Theoretical weight: 29.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
DNA polymerase delta subunit 4 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 19 amino acids
Theoretical weight: 2.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HCU8 (Residues: 1-19; Coverage: 18%)
Gene names: POLD4, POLDS

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 71.965Å b: 83.888Å c: 154.982Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Structure of the processive human Pol δ holoenzyme.
Lancey et al. (2020)
3 more

4 mentions without citation

Human PCNA Structure, Function and Interactions.
González-Magaña et al. (2020)
3 more