› 6hwl

X-ray diffraction
2.15Å resolution

Glucosamine kinase in complex with glucosamine, ADP and inorganic phosphate

Released:
DOI: 10.2210/pdb6hwl/pdb
PDB entry 6hwl coloured by chain, front view.
PDB entry 6hwl coloured by chain, side view.
PDB entry 6hwl coloured by chain, top view.
Source organism: Streptacidiphilus jiangxiensis
Primary publication:
Molecular Fingerprints for a Novel Enzyme Family in Actinobacteria with Glucosamine Kinase Activity.
Manso JA, Nunes-Costa D, Macedo-Ribeiro S, Empadinhas N, Pereira PJB
mBio 10 (2019)
PMID: 31088917

Function and Biology Details

Reaction catalysed: 
ATP + D-glucosamine = ADP + D-glucosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosamine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 451 amino acids
Theoretical weight: 48.31 KDa
Source organism: Streptacidiphilus jiangxiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1H7TQR5 (Residues: 1-438; Coverage: 100%)
Gene name: SAMN05414137_114149

Ligands and Environments

7 bound ligands:
Ligand CL 2 x CL
Ligand BTB 1 x BTB
Ligand PEG 3 x PEG
Ligand ADP 1 x ADP
Ligand MG 2 x MG
Ligand PO4 1 x PO4
Ligand GCS 1 x GCS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21
Unit cell:
a: 58.764Å b: 97.435Å c: 80.188Å
α: 90° β: 107.42° γ: 90°
R-values:
R R work R free
0.209 0.207 0.243
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Carbohydrate Metabolism in Bacteria: Alternative Specificities in ADP-Glucose Pyrophosphorylases Open Novel Metabolic Scenarios and Biotechnological Tools.
Bhayani et al. (2022)

1 mention without citation

Molecular Fingerprints for a Novel Enzyme Family in Actinobacteria with Glucosamine Kinase Activity.
Manso et al. (2019)