› 6hxa

X-ray diffraction
1.85Å resolution

AntI from P. luminescens catalyses terminal polyketide shortening in the biosynthesis of anthraquinones

Released:
DOI: 10.2210/pdb6hxa/pdb
PDB entry 6hxa coloured by chain, front view.
PDB entry 6hxa coloured by chain, side view.
PDB entry 6hxa coloured by chain, top view.
Source organism: Photorhabdus luminescens
Primary publication:
Molecular mechanism of polyketide shortening in anthraquinone biosynthesis of Photorhabdus luminescens.
Zhou Q, Bräuer A, Adihou H, Schmalhofer M, Saura P, Grammbitter GLC, Kaila VRI, Groll M, Bode HB
Chem Sci 10 6341-6349 (2019)
PMID: 31341589

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 45.78 KDa
Source organism: Photorhabdus luminescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7MZT8 (Residues: 1-384; Coverage: 100%)
Gene name: plu4186
Sequence domains: Esterase FrsA-like

Ligands and Environments

2 bound ligands:
Ligand PGE 1 x PGE
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 54.11Å b: 154.34Å c: 91.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 citation in other articles

Genome-enabled discovery of anthraquinone biosynthesis in Senna tora.
Kang et al. (2020)
7 more

1 mention without citation

Molecular mechanism of polyketide shortening in anthraquinone biosynthesis of Photorhabdus luminescens.
Zhou et al. (2019)