› 6hxh

X-ray diffraction
3.3Å resolution

Structure of the human ATP citrate lyase holoenzyme in complex with citrate, coenzyme A and Mg.ADP

Released:
DOI: 10.2210/pdb6hxh/pdb
PDB entry 6hxh coloured by chain, front view.
PDB entry 6hxh coloured by chain, side view.
PDB entry 6hxh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle.
Verschueren KHG, Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
Nature 568 571-575 (2019)
PMID: 30944476

Function and Biology Details

Reaction catalysed: 
ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156801 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-citrate synthase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 1050 amino acids
Theoretical weight: 115.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53396 (Residues: 1-424, 488-1101; Coverage: 94%)
Gene name: ACLY
Sequence domains:

Ligands and Environments


Cofactor: Ligand COA 8 x COA
4 bound ligands:
Ligand MG 16 x MG
Ligand PO4 8 x PO4
Ligand FLC 8 x FLC
Ligand ADP 8 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 150.364Å b: 154.011Å c: 154.087Å
α: 91.53° β: 110.04° γ: 107.46°
R-values:
R R work R free
0.157 0.156 0.187
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Mitochondrial Dysfunction and Oxidative Stress in Alzheimer's Disease.
Misrani et al. (2021)
16 more

2 mentions without citation

ACLY Nuclear Translocation in Human Macrophages Drives Proinflammatory Gene Expression by NF-κB Acetylation.
Santarsiero et al. (2021)
1 more