› 6hxn

X-ray diffraction
1.7Å resolution

Structure of the citryl-CoA lyase core module of Chlorobium limicola ATP citrate lyase (space group P3121)

Released:
DOI: 10.2210/pdb6hxn/pdb
PDB entry 6hxn coloured by chain, front view.
PDB entry 6hxn coloured by chain, side view.
PDB entry 6hxn coloured by chain, top view.
Source organism: Chlorobium limicola
Primary publication:
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle.
Verschueren KHG, Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
Nature 568 571-575 (2019)
PMID: 30944476

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
citrate synthase (unknown stereospecificity) Chains: A, B
Molecule details ›
Chains: A, B
Length: 279 amino acids
Theoretical weight: 30.76 KDa
Source organism: Chlorobium limicola
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9AJC4 (Residues: 351-608; Coverage: 42%)
Gene name: aclA
Sequence domains: Citrate synthase, C-terminal domain

Ligands and Environments

2 bound ligands:
Ligand COA 2 x COA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P3121
Unit cell:
a: 110.029Å b: 110.029Å c: 92.749Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.173 0.192
Expression system: Escherichia coli BL21

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Citations

17 review citations

Mitochondrial Dysfunction and Oxidative Stress in Alzheimer's Disease.
Misrani et al. (2021)
16 more